mouse anti sam68 primary antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology mouse anti sam68 primary antibody
Mouse Anti Sam68 Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sam68+antibody/Sam+68/pm41725810-106-30-34
Average 92 stars, based on 249 article reviews

mouse anti sam68 primary antibody - by Bioz Stars, 2026-10

92/100 stars

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Incubation:

Article Title: Clinical significance and effect of Sam68 expression in gastric cancer
Article Snippet: .. The slides were incubated with anti-Sam68 antibody (dilution, 1:50; cat. no. sc-4249; Santa Cruz Biotechnology, Inc.) in a humidified chamber at 4°C overnight. .. Following additional washing with PBS three times, the sections were sequentially incubated with HRP-conjugated secondary antibody (dilution, 1:100; cat. no. sc-6772; Santa Cruz Biotechnology, Inc.) at 37°C for 30 min and then washed three times with PBS.

Article Title: Sam68 Promotes NF-κB Activation and Apoptosis Signaling in Articular Chondrocytes during Osteoarthritis.
Article Snippet: Objectives To investigate the expression of Sam68 in articular cartilage of knee osteoarthritis (OA) and the relationship between Sam68 and NF-jB activation and apoptosis signaling in OA articular chondrocytes.. Methods Sam68 expression in normal and osteoarthritic cartilage was assessed by immunohistochemistry and RTPCR on both meniscal/ligamentous injury (MLI)-induced OA rat model and the clinical human OA cartilage tissues.. Sam68 expression in chondrocytes under tumor necrosis factor-alpha (TNF-a) stimuli was also assessed by immunoblot.

Article Title: Effective targeting of breast cancer stem cells by combined inhibition of Sam68 and Rad51.
Article Snippet: .. For immunoprecipitation experiments, an equal amount of protein lysates was incubated overnight at 4 °C with 2 μg of anti-Sam68 antibody (H-4, mouse IgG1, Santa Cruz Biotechnology) mixed with protein G-Sepharose (SigmaAldrich). ..

Article Title: Clinical significance and effect of Sam68 expression in gastric cancer.
Article Snippet: .. The slides were incubated with anti-Sam68 antibody (dilution, 1:50; cat. no. sc‐4249; Santa Cruz Biotechnology, Inc.) in a humidified chamber at 4 ̊C overnight. .. Following additional washing with PBS three times, the sections were sequentially incubated with HRP-conjugated secondary antibody (dilution, 1:100; cat. no. sc‐6772; Santa Cruz Biotechnology, Inc.) at 37 ̊C for 30 min and then washed three times with PBS.

other:

Article Title: Long non-coding RNA INXS is a critical mediator of BCL-XS induced apoptosis
Article Snippet: For BCL-XS and BCL-XL proteins detection, we incubated cell lysates from 106 cells in 100 μl RIPA buffer with 30 μl of protein A-sepharose 4B conjugate suspension (101042, Invitrogen) for 1 h, discarded the resin, and incubated the supernatant overnight with 1 μl antiBCL-X antibody (556361, BD Pharmingen) and 50 μl of protein A-sepharose 4B conjugate suspension (101042, Invitrogen).

Article Title: Effective targeting of breast cancer stem cells by combined inhibition of Sam68 and Rad51
Article Snippet: Stainings were analyzed using a confocal microscope (Nikon D-Eclipse C1).

Immunoprecipitation:

Western Blot:

Article Title: Clinical significance of Sam68 expression in endometrial carcinoma.
Article Snippet: Sam68 (Src-associated in mitosis of 68 kDa) is a substrate for tyrosine kinase c-Src during mitosis.. The nuclear protein level has been found to be associated with progression and prognosis in various human malignant tumors.. The aim of this study is to investigate the clinical value of Sam68 in endometrial carcinoma (EC).

Review